ENZYME METHOD FOR DETERMINATION OF ALPHA-LINKED GLUCOSE POLYMERS IN BIOLOGICAL MATERIALS

被引：400

作者：

MACRAE, JC

ARMSTRONG, DG

机构：

[1] Department of Agricultural Biochemistry, University of Newcastle upon Tyne

[2] Plant Chemistry Division, D.S.I.R, Palmerston North

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1968年 / 19卷 / 10期

关键词：

D O I：

10.1002/jsfa.2740191006

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A review of methods available for the determination of starch suggested, for reasons given, that none was satisfactory for the determination of α‐linked glucose polymers in biological materials. Therefore, a method was devised in which the specific hydrolysis of starch completely to glucose is achieved by means of an amyloglucosidase. Glucose is estimated using glucose oxidase. Contents of α‐linked glucose polymers in a number of biological samples were determined, and compared with those obtained using 0.36 N‐H2SO4 to effect hydrolysis. Copyright © 1968 John Wiley & Sons, Ltd

引用

页码：578 / +

页数：1

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