CHARACTERIZATION OF HYBRIDS BETWEEN BOVINE (MDBK) AND MOUSE (L-CELL) CELL-LINES

被引：4

作者：

CHINCHAR, VG ^{[1
]}

FLOYD, AD ^{[1
]}

CHINCHAR, GD ^{[1
]}

TAYLOR, MW ^{[1
]}

机构：

[1] INDIANA UNIV,PROGRAM MED SCI,BLOOMINGTON,IN 47401

来源：

BIOCHEMICAL GENETICS | 1979年 / 17卷 / 1-2期

关键词：

bovine hybrid cells; hypoxanthine guanine phosphoribosyltransferase (HGPRT); mengovirus; mouse hybrid cells;

D O I：

10.1007/BF00484479

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-deficient mutants of a bovine kidney cell line (MDBK) were selected following mutagenesis with ethylmethane sulfonate or ICR-170G. MDBK mutants were hybridized to thymidine kinase-deficient L cells and selected in HAT medium. Parental and hybrid cells were characterized for isozyme patterns of lactic dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, and glutamate oxalate transaminase. Chromosomes of MDBK can be distinguished from mouse L cells by configuration and by fluorescent staining with Hoechst 33-258 stain. Hybrid cells contained both MDBK and L-cell chromosomes and had elevated DNA content. MDBK cells are normally restrictive for mengovirus replication. Both permissive and restrictive hybrids were found. Our data indicate that there was preferential loss of MDBK chromosomes in the hybrid cell lines. © 1979 Plenum Publishing Corporation.

引用

页码：133 / 148

页数：16

共 26 条

[1]

[Anonymous], 1976, ZONE ELECTROPHORESIS

[2] EFFICIENT INEFFICIENT AND ABORTIVE INFECTION OF DIFFERENT MAMMALIAN CELLS BY SMALL RNA VIRUSES [J].