SIMPLE METHOD FOR RAPID PURIFICATION OF AVIAN-SARCOMA VIRUS SUPERCOILED DNA BY SELECTIVE PRECIPITATION OF INFECTED CELL CHROMATIN

被引：2

作者：

GUNTAKA, RV

机构：

[1] Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 90卷 / 01期

关键词：

D O I：

10.1016/0003-2697(78)90030-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and effective method to partially purify virus-specific DNA from avian sarcoma virus-infected QT6 cells has been developed. This method consists of lysing infected cell nuclei in water followed by precipitation of the resulting chromatin with sodium chloride. More than 98% of the host cell DNA could be removed by this method without diminishing viral DNA yields. This method is equally applicable to SV40 DNA purification. © 1978.

引用

页码：256 / 261

页数：6

共 6 条

[1] BENPORAT T, 1976, VIROLOGY, V69, P560
[2] PRO-VIRAL DNA OF MOLONEY LEUKEMIA-VIRUS - PURIFICATION AND VISUALIZATION
GIANNI, AM
HUTTON, JR
SMOTKIN, D
WEINBERG, RA
[J]. SCIENCE, 1976, 191 (4227) : 569 - 571
[3] COVALENTLY CLOSED CIRCULAR DNA OF AVIAN-SARCOMA VIRUS - PURIFICATION FROM NUCLEI OF INFECTED QUAIL TUMOR-CELLS AND MEASUREMENT BY ELECTRON-MICROSCOPY AND GEL-ELECTROPHORESIS
GUNTAKA, RV
RICHARDS, OC
SHANK, PR
KUNG, HJ
DAVIDSON, N
FRITSCH, E
BISHOP, JM
VARMUS, HE
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1976, 106 (02) : 337 - 357
[4] ETHIDIUM-BROMIDE INHIBITS APPEARANCE OF CLOSED CIRCULAR VIRAL DNA AND INTEGRATION OF VIRUS-SPECIFIC DNA IN DUCK CELLS INFECTED BY AVIAN-SARCOMA VIRUS
GUNTAKA, RV
MAHY, BWJ
BISHOP, JM
VARMUS, HE
[J]. NATURE, 1975, 253 (5492) : 507 - 511
[5] SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES
HIRT, B
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1967, 26 (02) : 365 - &
[6] ISOLATION OF HERPES-SIMPLEX VIRUS-DNA FROM HIRT SUPERNATANT
PATER, MM
HYMAN, RW
RAPP, F
[J]. VIROLOGY, 1976, 75 (02) : 481 - 483

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