RECOMBINANT E-SELECTIN-PROTEIN MEDIATES TUMOR-CELL ADHESION VIA SIALYL-LEA AND SIALYL-LEX

被引:98
作者
MAJURI, ML [1 ]
MATTILA, P [1 ]
RENKONEN, R [1 ]
机构
[1] UNIV HELSINKI,DEPT BACTERIOL & IMMUNOL,HAARTMANINKATU 3,SF-00290 HELSINKI 29,FINLAND
关键词
D O I
10.1016/0006-291X(92)91885-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
E-selectin (previously known as ELAM-1) is one of the adhesion molecules expressed on activated endothelium. Here we show that HL-60 cells express sialyl-Lex, but not Sialyl-Lea on their surface, a colon carcinoma cell line COLO 205 express both these epitopes and another colon carcinoma COLO 320 does not express either one of them. HL-60 and COLO 205 cell adhere strongly to E-selectin coated microwells, whereas COLO 320 does not adhere at all to E-selectin. Finally we provide evidence that monoclonal anti-sialyl-Lex can abolish part of the adherence of HL-60 cells to recombinant E-selectin. The adherence of COLO 205 cells can be decreased by either monoclonal anti-sialyl-Lea or anti-sialyl-Lex antibodies. These results indicate that cell-associated sialylated carbohydrate moieties can act as ligands for recombinant E-selectin. © 1992.
引用
收藏
页码:1376 / 1382
页数:7
相关论文
共 23 条
  • [1] BEJAMIN C, 1990, BIOCHEM BIOPH RES CO, V171, P348
  • [2] BERG EL, 1991, J BIOL CHEM, V266, P14869
  • [3] SELECTINS - A FAMILY OF ADHESION RECEPTORS
    BEVILACQUA, M
    BUTCHER, E
    FURIE, B
    FURIE, B
    GALLATIN, M
    GIMBRONE, M
    HARLAN, J
    KISHIMOTO, K
    LASKY, L
    MCEVER, R
    PAULSON, J
    ROSEN, S
    SEED, B
    SIEGELMAN, M
    SPRINGER, T
    STOOLMAN, L
    TEDDER, T
    VARKI, A
    WAGNER, D
    WEISSMAN, I
    ZIMMERMAN, G
    [J]. CELL, 1991, 67 (02) : 233 - 233
  • [4] IDENTIFICATION OF AN INDUCIBLE ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE
    BEVILACQUA, MP
    POBER, JS
    MENDRICK, DL
    COTRAN, RS
    GIMBRONE, MA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) : 9238 - 9242
  • [5] ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS
    BEVILACQUA, MP
    STENGELIN, S
    GIMBRONE, MA
    SEED, B
    [J]. SCIENCE, 1989, 243 (4895) : 1160 - 1165
  • [6] CARBOHYDRATE LIGANDS OF THE LEC CELL-ADHESION MOLECULES
    BRANDLEY, BK
    SWIEDLER, SJ
    ROBBINS, PW
    [J]. CELL, 1990, 63 (05) : 861 - 863
  • [7] CARLOS T, 1991, BLOOD, V77, P2266
  • [8] Cell-cell adhesion and membrane glycosylation
    Feizi, Ten
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1991, 1 (05) : 766 - 770
  • [9] FUKUSHIMA K, 1984, CANCER RES, V44, P5279
  • [10] POSSIBLE FUNCTIONS OF TUMOR-ASSOCIATED CARBOHYDRATE ANTIGENS
    HAKOMORI, S
    [J]. CURRENT OPINION IN IMMUNOLOGY, 1991, 3 (05) : 646 - 653