METAL-MEDIATED PROTEIN STABILIZATION

被引：76

作者：

ARNOLD, FH

ZHANG, JH

机构：

[1] Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena

来源：

TRENDS IN BIOTECHNOLOGY | 1994年 / 12卷 / 05期

关键词：

D O I：

10.1016/0167-7799(94)90081-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Metal binding provides several useful routes to protein stabilization. Metal chelation by binding sites engineered into proteins can increase thermal stability, conformational stability and reduce proteolytic degradation. As few as two coordinating ligands, if properly positioned on the protein surface, can form chelating sites suitable for metal binding and stabilization. Thus, this approach is relatively easy to implement and can be applied to a wide range of proteins.

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页码：189 / 192

页数：4

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