DETECTION OF POLY(U) HYBRIDIZATION USING AZIDO MODIFIED POLY(A)-COATED PIEZOELECTRIC-CRYSTALS

被引：12

作者：

CAMPBELL, NF ^{[1
]}

EVANS, JA ^{[1
]}

FAWCETT, NC ^{[1
]}

机构：

[1] UNIV SO MISSISSIPPI,DEPT CHEM & BIOCHEM,HATTIESBURG,MS 39406

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2328

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique has been developed which uses AT-cut, 9 MHz piezoelectric crystals for the immobilization of single stranded nucleic acids and the subsequent hybridization reactions. This hybridization technique is based on detecting small changes in mass on the surface of a piezoelectric crystal which occur upon immobilizing azido containing probe nucleic acid on the surface and hybridizing complementary target nucleic acid to previously immobilized probe. The immobilized probe could be used for repeated hybridization tests. The hybridization assays were performed in solution and the frequency measurements were performed in the dry state. © 1993 Academic Press, Inc.

引用

页码：858 / 863

页数：6

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