ALGINATE LYASES OF PSEUDOMONADS

被引:35
作者
KASHIWABARA, Y
SUZUKI, H
NISIZAWA, K
机构
[1] Botanical Institute, Tokyo Kyoiku University, Otsuka, Tokyo
关键词
D O I
10.1093/oxfordjournals.jbchem.a129175
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two kinds of pseudomonads, Nos. 8 and 9 were isolated from sea sand and decaying algal fronds. Crude extracts from these bacterial cells grown on an alginate medium and the partially purified enzyme preparations obtained therefrom degraded eliminatively various substrates such as commercial alginate, mannuronic acid-rich (M-rich)and guluronic acid-rich (G-rich) alginates, a short-chain polyguluronide with DP¯ 15 (SG) and its reduced derivative and a short-chain polymannuronide with DP¯ 11 (SM) in different manners. However, a highly purified lyase preparation from Pseudomonas No. 9 showed the activity for SG but not for SM, and the independent existence of a polyguluronide lyase" was ascertained. © 1969 BY THE JOURNAL OF BIOCHEMISTRY."
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页码:503 / +
页数:1
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