CHROMATOGRAPHY OF P-32-LABELLED OLIGONUCLEOTIDES ON THIN LAYERS OF DEAE-CELLULOSE

被引：485

作者：

BROWNLEE, GG

SANGER, F

机构：

[1] M.R.C. Laboratory of Molecular Biology, Cambridge, CB2 2QH, Hills Road

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00786.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A two‐dimensional fractionation procedure has been developed for separating radioactively‐labelled oligonucleotides of up to 50 residues long, using uniformly 32P‐labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate at pH 3.5 in the first dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE‐cellulose and cellulose in the second dimension. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：395 / &

共 7 条

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[7] SANGER F., 1965, J MOL BIOL, V13, P373

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