POINT SUBSTITUTIONS IN A PROMOTER-LIKE REGION AND THE V1 GENE AFFECT THE HOST RANGE AND SYMPTOMS OF MAIZE STREAK VIRUS

被引:48
作者
BOULTON, MI
KING, DI
DONSON, J
DAVIES, JW
机构
[1] The John Innes Institute, The John Innes Centre for Plant Science Research, Norwich, NR4 7UN, Colney Lane
关键词
D O I
10.1016/0042-6822(91)90124-T
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nucleotide sequences of full-length infectious clones of two symptomatic and host range variants (MSV-Ns and MSV-Nm) of the Nigerian strain of maize streak virus (MSV) have been determined and shown to differ by only three nucleotides. MSV-Ns produced symptoms in infected maize plants sooner and the streaks were wider and more chlorotic than those of MSV-Nm; variant MSV-Ns also had a wider host range within the Gramineae. None of the three nucleotide differences resulted in amino acid changes. Site-directed mutagenesis showed that a substitution at nucleotide (nt) 40 in the V1 gene affected streak width, while severity of chlorosis, length of streaks, latency, and host range was determined by a single base change at nt 2473 in the large intergenic region. The nt 2473 change altered a potential promoter sequence (TATA box) in MSV-Ns 101 nucleotides upstream of the initiation codon of the C1 gene. Mutagenesis of TATA sequences located downstream of TATA -101 showed that TATA -101 alone was sufficient to confer a wide host range phenotype on MSV-Ns and suggested that it might function as a promoter for the expression of complementary-sense open reading frames. When compared with an updated promoter consensus derived from genes of the Gramineae, the promoter context around TATA -101 in MSV-Ns was not more favorable than those found at -57 and -62 in MSV-Nm. © 1991.
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页码:114 / 121
页数:8
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