ENDOTHELIUM STIMULATING FACTOR FROM WALKER CARCINOMA-CELLS - RELATION TO TUMOR ANGIOGENIC FACTOR

A low molecular weight endothelial cell stimulating factor from Walker carcinoma cells has been partially purified. In its physico-chemical properties and the response it elicits in endothelial cells it is similar to, or identical with, an endothelial stimulating factor (ESF) in normal bovine parotid glands. The partially purified factor did not promote proliferation of cultured endothelial cells but when injected into mice it caused endothelial cell hypertrophy and neovascularization-properties characteristic of the response to tumour angiogenic factor. The molecular weight of the factor estimated by gel diffusion chromatography is 210. © 1979.