EVALUATION OF THE INVITRO PRODUCTION OF TUMOR-NECROSIS-FACTOR BY MONOCYTES IN DIALYSIS PATIENTS

被引：15

作者：

RYAN, J ^{[1
]}

BEYNON, H ^{[1
]}

REES, AJ ^{[1
]}

CASSIDY, MJD ^{[1
]}

机构：

[1] ROYAL POSTGRAD MED SCH,DEPT MED,LONDON W12 0HS,ENGLAND

来源：

BLOOD PURIFICATION | 1991年 / 9卷 / 03期

关键词：

DIALYSIS; TUMOR NECROSIS FACTOR; RENAL FAILURE;

D O I：

10.1159/000170010

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Modification of the cellular immune response in uraemia is partly responsible for the increased susceptibility to infection found in dialysis patients. In order to study this further we have evaluated the in vitro production of tumour necrosis factor (TNF) by peripheral-blood monocytes (PBMCs) to stimulation by lipopolysaccharide (LPS) from dialysis patients with end-stage renal failure. The patients were subdivided into two groups according to the type of dialysis: those undergoing haemodialysis (HD; n = 12) and continuous ambulatory peritoneal dialysis (CAPD; n = 18). Results were compared with those of controls taken from healthy laboratory staff (n = 7). The experiments show that the secretion of TNF by PBMCs in response to LPS is significantly augmented in patients undergoing HD when compared to those on CAPD (81.3 +/- 38.7 vs. 18.2 +/- 13.3 U/ml, mean +/- SD, p < 0.001) and controls (81.3 +/- 38.7 vs. 18.1 +/- 6.6 U/ml, p < 0.001). There was no significant difference between the CAPD group and controls. In vitro production of TNF fell slightly following a single HD session (81.3 +/- 38.7 U/ml before HD and 50.5 +/- 28.7 U/ml after HD, p < 0.05). We conclude from this study that TNF release from PBMCs is augmented in patients with chronic renal failure receiving chronic HD but not in a similar group receiving CAPD, in vitro. TNF release, however, is suppressed immediately following a single HD session. We suggest that HD rather than uraemia per se up-regulates monocyte secretion of TNF in vitro and that this is not an immediate response to activation by membrane polymer.

引用

页码：142 / 147

页数：6

共 21 条

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