VOLTAMMETRIC STUDIES OF A PSYCHOTROPIC-DRUG WITH NITRO-GROUPS - DETERMINATION OF FLUNITRAZEPAM IN URINE USING HMDE

被引：20

作者：

BERMEJO, E ^{[1
]}

ZAPARDIEL, A ^{[1
]}

PEREZ, JA ^{[1
]}

HUERTA, A ^{[1
]}

HERNANDEZ, L ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID, DEPT ANALYT CHEM & INSTRUMENTAL ANAL, E-28049 MADRID, SPAIN

来源：

TALANTA | 1993年 / 40卷 / 11期

关键词：

D O I：

10.1016/0039-9140(93)80080-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The adsorption behaviour of flunitrazepam at the hanging mercury drop electrode was studied by staircase voltammetry and by adsorptive stripping differential pulse voltammetry. Flunitrazepam is adsorbed in the whole potential range, from the most positive values up to the reduction potential. Flunitrazepam reduction product is also adsorbed. The time dependence of the voltammetric response proves that a diffusion-controlled adsorption takes place. Flunitrazepam can be determined (down to nanomolar levels) by using adsorptive preconcentration prior to the differential pulse voltammetric scan. An application of such a method to flunitrazepam determination in human urine is described. The detection limit was 30 ng per milliliter of urine with a 20-sec accumulation time; the mean relative standard deviation was lower than 3.2% and the mean recovery 97.8%.

引用

页码：1649 / 1656

页数：8

共 90 条

[1]

BAKAVDI M, 1984, J CHROMATOGR, V229, P465

[2]

BARBATO F, 1980, SOC ITAL BIOL SPER, V56, P879

[3] SIMULTANEOUS HPLC ANALYSIS OF THE HYPNOTIC BENZODIAZEPINES NITRAZEPAM, ESTAZOLAM, FLUNITRAZEPAM, AND TRIAZOLAM IN PLASMA [J].

BOUKHABZA, A ;

LUGNIER, AAJ ;

KINTZ, P ;

MANGIN, P .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1991, 15 (06) :319-322

[4] PHARMACOKINETICS OF FLUNITRAZEPAM FOLLOWING SINGLE-DOSE AND MULTIPLE-DOSE ORAL-ADMINISTRATION TO HEALTHY HUMAN SUBJECTS [J].

BOXENBAUM, HG ;

POSMANTER, HN ;

MACASIEB, T ;

GEITNER, KA ;

WEINFELD, RE ;

MOORE, JD ;

DARRAGH, A ;

OKELLY, DA ;

WEISSMAN, L ;

KAPLAN, SA .

JOURNAL OF PHARMACOKINETICS AND BIOPHARMACEUTICS, 1978, 6 (04) :283-293

[5]

CANO JP, 1977, ARZNEIMITTELFORSCH, V27, P338

[6] PHOTOCHEMICAL ACTIVITY OF 7-NITRO-I,4-BENZODIAZEPINES - FORMATION OF SINGLET MOLECULAR-OXYGEN, ISOLATION AND IDENTIFICATION OF DECOMPOSITION PRODUCTS [J].

CORNELISSEN, PJG ;

VANHENEGOUWEN, GMJ .

PHARMACEUTISCH WEEKBLAD-SCIENTIFIC EDITION, 1981, 3 (03) :800-809

[7]

Corti P, 1987, Boll Chim Farm, V126, P376

[8]

DALDRAP T, 1982, J CHROMATOGR, V244, P115

[9] COMBINATION OF TLC, GLC (OV1 AND OV17) AND HPLC (RP18) FOR A RAPID DETECTION OF DRUGS AND RELATED-COMPOUNDS [J].

DALDRUP, T ;

SUSANTO, F ;

MICHALKE, P .

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1981, 308 (05) :413-427

[10] HYDROLYSIS OF CLONAZEPAM, FLUNITRAZEPAM AND NITRAZEPAM BY HYDROCHLORIC-ACID - IDENTIFICATION OF SOME ADDITIONAL PRODUCTS [J].

DEBRUYNE, MMA ;

SINNEMA, A ;

VERWEIJ, AMA .

FORENSIC SCIENCE INTERNATIONAL, 1984, 24 (02) :125-135

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