IMMUNOHISTOCHEMICAL STUDY OF THE DISTRIBUTION OF INTERCELLULAR-ADHESION MOLECULE-1 AND LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1 IN CHRONIC TYPE-B HEPATITIS

被引：18

作者：

DOI, T

YAMADA, G

MIZUNO, M

TSUJI, T

机构：

[1] The First Department of Internal Medicine, Okayama University Medical School, Okayama, 700

来源：

JOURNAL OF GASTROENTEROLOGY | 1994年 / 29卷 / 02期

关键词：

ADHESION MOLECULE; INTERCELLULAR ADHESION MOLECULE-1; LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; HEPATITIS B VIRUS;

D O I：

10.1007/BF02358678

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) in the livers of 11 patients with chronic hepatitis B was studied immunohistochemically by light and electron microscopy to clarify the role of these adhesion molecules in tissue damage in chronic hepatitis B. On hepatocytes, ICAM-1 expression was confined to the bile canalicular surface when the liver inflammation was mild. In contrast, when the liver inflammation was severe, ICAM-1 was distributed on the entire surface of the hepatocyte, including the sinusoidal and lateral membranes; lymphocytes which were mostly positive for LFA-1, were often observed invading deeply among these hepatocytes. The degree of ICAM-1 expression on the hepatocytes was also related to the expression of HLA class 1 antigen. In liver showing diffuse expression of ICAM-1 on the hepatocytes, strong expression of HLA class 1 antigen was observed, and amounts of HBV in the liver were decreased. Diffuse expression of ICAM-1 and HLA class 1 antigen was mostly observed after acute exacerbation of liver inflammation. These results suggest that the ICAM-1/LFA-1 pathway is involved in the immunological mechanism, responsible for liver cell damage in chronic hepatitis B. © 1994 Springer-Verlag.

引用

页码：164 / 171

页数：8

共 26 条

[1]

Mondelli M., Mieli-Vergani G., Alberti A., Et al., Specificity of T Lymphocyte cytotoxity to autologous hepatocytes in chronic hepatitis B virus infection: evidence that T cells are directed against HBV core antigen expressed on hepatocytes, J Immunol, 129, pp. 2773-2778, (1982)

[2]

Yamada G., Nishihara T., Hyodo I., Et al., Cellular immune response in liver of patients with chronic hepatitis B: Electron microscopic observation of lymphocyte subsets by the immunoperoxidase method using monoclonal antibodies, Gastroenterol Jpn, 19, pp. 517-529, (1984)

[3]

Hyodo I., Yamada G., Nagashima H., Cellular immune responses in acute exacerbation of hepatitis Be antigen-positive type B chronic hepatitis, Gastroenterol Jpn, 20, pp. 315-323, (1985)

[4]

Manabe K., Yamada G., Nagashima H., Immunohistochemical study of HLA class 1 antigens on the hepatocytes of patients with chronic hepatitis B, Gastroenterol Jpn, 21, pp. 357-364, (1986)

[5]

Barnaba V., Franco A., Alberti A., Et al., Recognition of hepatitis B virus envelope proteins by liver-infiltrating T lymphocytes in chronic HBV infection, J Immunol, 143, pp. 2650-2655, (1989)

[6]

Milich D.R., McLachlan A., Moriarty A., Et al., Immune response to hepatitis B virus core antigen (HBcAg): Localization of T cell recognition sites within HBcAg/HBeAg, J Immunol, 139, pp. 1227-1231, (1987)

[7]

Milich D.R., Hughes J.L., McLachlan A., Et al., Hepatitis B synthetic immunogen comprised of nucleocapsid T cell site and an envelope B cell epitope, Proc Natl Acad Sci USA, 85, pp. 1610-1614, (1988)

[8]

Ou J.H., Laub O., Rutter W.J., Hepatitis B virus gene function: The precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen, Proc Natl Acad Sci USA, 83, pp. 1578-1582, (1986)

[9]

Yamada G., Takaguchi K., Matsueda K., Et al., Immunoelectron microscopic observation of intrahepatic HBeAg in patients with chronic hepatitis B, Hepatology, 12, pp. 133-140, (1990)

[10]

Springer T.A., Dustin M.L., Kishimoto T.K., Et al., The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: Cell adhesion receptors of the immune system, Annu Rev Immunol, 5, pp. 223-252, (1987)

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