ELECTRON-MICROSCOPIC ANALYSIS OF IN-VITRO REPLICATION PRODUCTS OF ORS-8, A MAMMALIAN ORIGIN ENRICHED SEQUENCE

被引：20

作者：

PEARSON, CE

SHIHABELDEEN, A

PRICE, GB

ZANNISHADJOPOULOS, M

机构：

[1] McGill Cancer Centre, Department of Medicine, McGill University, Montreal, H3G 1Y6, Quebec

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1994年 / 20卷 / 03期

关键词：

D O I：

10.1007/BF02254755

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscopy was used to map the initiation site of ors 8 DNA replication in vitro in a system that is capable of initiating and supporting one round of semiconservative replication of cloned mammalian DNA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the monkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.

引用

页码：147 / 152

页数：6

共 25 条

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