SHEAR-STRESS INDUCES CHANGES IN THE MORPHOLOGY AND CYTOSKELETON ORGANIZATION OF ARTERIAL ENDOTHELIAL-CELLS

被引：52

作者：

CUCINA, A ^{[1
]}

STERPETTI, AV ^{[1
]}

PUPELIS, G ^{[1
]}

FRAGALE, A ^{[1
]}

LEPIDI, S ^{[1
]}

CAVALLARO, A ^{[1
]}

GIUSTINIANI, Q ^{[1
]}

DANGELO, LS ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,SCH MED,DEPT SURG 1,ROME,ITALY

来源：

EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY | 1995年 / 9卷 / 01期

关键词：

ENDOTHELIAL CELLS; CYTOSKELETON; SHEAR STRESS;

D O I：

10.1016/S1078-5884(05)80230-8

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Objectives: The aim of this study was to determine the charges in the morphology and cytoskeleton organisation of endothelial cells (EC) determined by exposure to a laminar flow. Cultured EC were exposed to a wall shear stress of 6 dyne/cm(2) for 24 hours. Chief outcome measures: The morphology of EC was analysed by light and scanning electron microscopy. The organisation of the cytoskeleton was determined by double fluorescence labeling with antibody anti-vementin, anti-vinculin, anti-tubulin, and with rhodamine-labeled phalloidin. Results: EC exposed to laminar flow become round-shaped with decreased areas of adhesion to the substrate. There was a clear reorganisation of the cytoskeleton after exposure to shear stress; the distribution of actin changed from a stress fibre pattern to a more diffuse membrane associated distribution. These changes in shape and cytoskeleton organisation were reversible after a 48-hour resting period. Conclusions: EC respond to laminar flow in a predictable manner and these findings may be correlated to the functional changes of EC observed after exposure to shear stress.

引用

页码：86 / 92

页数：7

共 28 条

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