EFFECT OF FILTRATION OF PLATELET CONCENTRATES ON THE ACCUMULATION OF CYTOKINES AND PLATELET-RELEASE FACTORS DURING STORAGE

Background: Platelet transfusions are frequently accompanied by febrile nonhemolytic transfusion reactions. These may be due, in part, to the release of cytokines interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor alpha (TNF-alpha) by white cells (WBCs) into the plasma during storage of platelet concentrates (PCs). Acting as endogenous pyrogens, these agents may induce inflammatory responses. Study Design and Methods:This study proposed to determine if WBC reduction in PCs by filtration significantly reduced the levels of cytokines normally generated during storage of unfiltered PCs up to 5 days. Serotonin, platelet-derived growth factor (PDGF-AB), and von Willebrand factor levels were also assessed to establish whether or not filtration or storage elicited significant platelet activation and granule release. Results: Filtration significantly reduced total WBC counts by 99.1 percent before storage (p<0.001) without affecting total platelet counts. Compared to unfiltered PCs, filtration prevented rise in the levels of each cytokine by Day 3 for IL-1 beta (27.7 vs. 0.6 pg/mL; p<0.05), IL-6 (114.2 vs. 0.4 pg/mL; p<0.001), and IL-8 (4.2 vs. 0.02 ng/mL; p<0.001). By Day 5, further increases in the levels of all cytokines were noted in unfiltered PCs, but Day 0 levels remained in filtered PCs (IL-1 beta: 105.4 vs. 0.4 pg/mL, p<0.001;TNF-alpha: 42.2 vs. 7.5 pg/mL, p<0.025; IL-6: 268.8 vs. 0.4 pg/mL, p<0.001; and IL-8: 7.6 vs. 0.02 ng/mL, p<0.001). From Day 0 to Day 5, there were significant increases in serotonin (21.3 vs. 6.3 ng/mL, p<0.05), PDGF-AB (72.6 vs. 25.8 ng/mL, p<0.001), and von Willebrand factor (4.7 vs. 2.7 IU/mL, p<0.05) in unfiltered PCs, with similar increased levels being observed in filtered PCs during storage. Conclusion: These data indicate that the accumulation of high levels of cytokines in stored PCs could be prevented by WBC-reduction filtration of PCs without the induction of significant platelet activation or granule release. As cytokines have the potential to induce febrile nonhemolytic transfusion reactions in patients, the transfusion of WBC-reduced PCs would be expected to reduce the frequency and severity of such reactions.