RAPID METHOD FOR THE ROUTINE DETERMINATION OF CAFFEINE AND ITS METABOLITES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：30

作者：

DOBROCKY, P

BENNETT, PN

NOTARIANNI, LJ

机构：

[1] UNIV BATH,SCH PHARM & PHARMACOL,CLAVERTON DOWN,BATH BA2 7AY,AVON,ENGLAND

[2] UNIV BATH,SCH POSTGRAD MED,BATH BA2 7AY,AVON,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)E0369-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Caffeine is a popular compound for phenotyping individuals for CYP4501A2, xanthine oxidase (XO) and N-acetyltransferase (NAT) utilising urinary metabolites. The analysis is complex since at least thirteen metabolites are excreted by man. Past methods have been less than satisfactory in that either not all the metabolites have been resolved and/or extractions selective for particular groups of metabolites are required prior to chromatography. We report a method for the rapid analysis of caffeine and metabolites in urine that negates the requirement for an extraction step, and also a method for plasma analysis.

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页码：104 / 108

页数：5

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