NMR ROTATING FRAME RELAXATION STUDIES OF INTRA-MOLECULAR MOTION IN PEPTIDES - TYROSINE RING MOTION IN METHIONINE-ENKEPHALIN

被引：21

作者：

BLEICH, HE ^{[1
]}

DAY, AR ^{[1
]}

FREER, RJ ^{[1
]}

GLASEL, JA ^{[1
]}

机构：

[1] MED COLL VIRGINIA,DEPT PHARMACOL,RICHMOND,VA 23298

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 04期

关键词：

D O I：

10.1016/S0006-291X(79)80027-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proton, rotating frame, nuclear magnetic spin-lattice relaxation measurements have been performed in aqueous solution on the unlabelled tyrosyl ring nuclei in [1-(α,ββ,δδ-2H5)-L-Tyr,5-L-Met]-enkephalin. In the temperature range measured, 7°C to 30°C, the chemical shift difference between the two epsilon protons is modulated by motions of the main chain and the ring with an average rate of the order of ∼ 400 sec.-1 This work confirms an earlier suggestion by us that the ring of the tyrosyl residue in Methionine-enkephalin is quite rigidly fixed with respect to the peptide main chain compared with overall molecular tumbling. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.

引用

页码：1146 / 1153

页数：8

共 20 条

[1] 360 MHZ PROTON NMR-SPECTRA OF ACTIVE AND INACTIVE DERIVATIVES OF METHIONINE-ENKEPHALIN - ASSIGNMENTS, DERIVED PARAMETERS, CONFORMATIONAL IMPLICATIONS [J].