DEVELOPMENT OF A BETA-GALACTOSIDASE ALPHA-COMPLEMENTATION SYSTEM FOR MOLECULAR-CLONING IN BACILLUS-SUBTILIS

被引：49

作者：

HAIMA, P

VANSINDEREN, D

SCHOTTING, H

BRON, S

VENEMA, G

机构：

[1] CTR BIOL SCI,DEPT GENET,KERKLAAN 30,9751 NN HAREN,NETHERLANDS

[2] CTR BIOL SCI,DEPT MICROBIOL,9751 NN HAREN,NETHERLANDS

来源：

GENE | 1990年 / 86卷 / 01期

关键词：

gene fusion; homologous recombination; lacZ gene; multiple cloning site; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90114-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A versatile β-galactosidase α-complementation system for Bacillus subtilis was developed, which can be used for molecular cloning in this Gram+ organism. The cloning system, which is based on the highly efficient host-vector system 6GM-pHP13, offers several advantages over previously described systems: (1) convenient direct selection of recombinants; (2) the cloning of large heterologous DNA fragments with high efficiency; and (3) the availability of six unique target sites: SphI, NdeI, NheI, BamHI, SmaI and EcoRI. © 1990.

引用

页码：63 / 69

页数：7

共 34 条

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