EFFECT OF HIGH-DENSITY CULTIVATION ON PLASMID COPY NUMBER IN RECOMBINANT ESCHERICHIA-COLI-CELLS

被引：17

作者：

HORN, U ^{[1
]}

KRUG, M ^{[1
]}

SAWISTOWSKI, J ^{[1
]}

机构：

[1] INST BIOTECHNOL, O-7050 LEIPZIG, GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 03期

关键词：

D O I：

10.1007/BF01026797

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The copy number of plasmid harboring E. coli K 12 strains was examined in fed batch cultivations in semisynthetic and synthetic media. Under conditions of high cell density (45-50 g dry weight/I) the plasmid copy number reached a maximum level between 200 and 400 copies per cell. A decrease of phosphate concentration in the medium was obtained similar to the increase of copy number. A high segregational and structural stability of vectors used in this work was observed. © 1990 Kluwer Academic Publishers.

引用

页码：191 / 196

页数：6

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