ISOLATION OF AN OUTER-MEMBRANE PROTEIN COMPLEX FROM BORRELIA-BURGDORFERI BY NORMAL-BUTANOL EXTRACTION AND HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY

被引：17

作者：

GONDOLF, KB

BATSFORD, SR

VOGT, A

机构：

[1] Department of Immunology, Institute for Medical Microbiology, D-7800 Freiburg

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 521卷 / 02期

关键词：

D O I：

10.1016/0021-9673(90)85056-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Borrelia burgdorferi, the causative agent of Lyme disease, expresses two major membrane proteins, designated outer surface proteins A and B, which are of antigenic relevance, especially in the chronic phase of Lyme disease. Both proteins exhibit strain-related molecular weight variation. A method is described for obtaining these proteins from the bacterial membrane, without the use of detergents, by a combination of n-butanol extraction and cation-exchange chromatography on a Mono S fast protein liquid chromatographic column. This method yields up to five times larger amounts of the proteins in aqueous solution than previously described protocols, which applied ionic or non-ionic detergents. A comparison of extracts obtained by this method from different Borrelia burgdorferi strains is reported. © 1990.

引用

页码：325 / 334

页数：10

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