IMPROVED METHOD FOR IR DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN

被引：379

作者：

BAXTER, A

DILLON, M

TAYLOR, KDA

ROBERTS, GAF

机构：

[1] NOTTINGHAM POLYTECH,DEPT CHEM & PHYS,BURTON ST,NOTTINGHAM,ENGLAND

[2] HUMBERSIDE POLYTECH,DEPT FOOD STUDIES,GRIMSBY,S HUMBERSIDE,ENGLAND

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1992年 / 14卷 / 03期

关键词：

CHITIN; CHITOSAN; N-ACETYLATION; IR SPECTROSCOPY;

D O I：

10.1016/S0141-8130(05)80007-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three published i.r. absorption band ratios for determining the % N-acetylation of chitosan have been used to follow the rate of alkaline deacetylation of chitin. Only one, the A 1655/A3450 ratio, gives the expected rate of deacetylation curve. Consideration of the various i.r. ratios has led to a new relationship, % N-acetylation = (A1655/A3450) x 115, where the absorbance of the amide I band at approximately 1655 cm-1 is determined using the baseline proposed previously. The values obtained using this proposed relationship agree closely with those obtained from dye adsorption measurements for chitosans having % N-acetylation values in the range 0-55%.

引用

页码：166 / 169

页数：4

共 18 条

[1] STUDIES ON CHITOSAN .1. DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN BY ULTRAVIOLET SPECTROPHOTOMETRY AND GEL-PERMEATION CHROMATOGRAPHY [J].