SENSITIVE DETERMINATION OF THE BENZENE METABOLITE S-PHENYLMERCAPTURIC ACID IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：30

作者：

EINIG, T

DEHNEN, W

机构：

[1] Medizinisches Institut für Umwelthygiene, Abteilung Biochemie, D-40225 Düsseldorf

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 697卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)00788-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for the determination of the specific benzene metabolite S-phenylmercapturic acid in urine. The analyte is determined by HPLC with fluorescence detection after solid-phase extraction of urine with C-18 material and hydrolysis followed by precolumn derivatization. The samples are separate by a column-switching method with a dual column system. As the method is highly sensitive (detection limit ca. 1 mu g/l), urinary S-phenylmercapturic acid concentrations for non-exposed persons (e.g., non-smokers) can also be measured precisely.

引用

页码：371 / 375

页数：5

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