CEL1 - A NOVEL CELLULOSE-BINDING PROTEIN SECRETED BY AGARICUS-BISPORUS DURING GROWTH ON CRYSTALLINE CELLULOSE

被引:20
作者
ARMESILLA, AL [1 ]
THURSTON, CF [1 ]
YAGUE, E [1 ]
机构
[1] KINGS COLL LONDON,DIV LIFE SCI,MICROBIOL GRP,LONDON W8 7AH,ENGLAND
关键词
AGARICUS BISPORUS; CELLULASE; CELLULOSE BINDING; FUSION PROTEIN; ANTIBODY; CEL1;
D O I
10.1016/0378-1097(94)90050-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The cell gene of Agaricus bisporus encodes a protein (CEL1) that has an architecture resembling the multi-domain fungal cellulases, although the sequence of its putative catalytic core is not matched by any other in the protein and nucleic acid data bases. The N-terminal half of the putative catalytic domain of CEL1 was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase. The fusion protein was used to raise a CEL1-specific antibody. CEL1 was detected as an extracellular 49.8 kDa protein in A. bisporus cellulose-grown cultures, where it bound strongly to cellulose. CEL1 was neither an endoglucanase, a cellobiohydrolase able to hydrolyze fluorogenic cellobiosides, a beta-glucosidase, a xylanase, nor a cellobiose:quinone oxidoreductase. CEL1 was present in some fractions of culture fluid separated by electrophoresis which released soluble sugars from crystalline cellulose.
引用
收藏
页码:293 / 299
页数:7
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