EVALUATION OF POLYMERIC MATERIALS BY CELL-CULTURE METHOD

A new evaluation method for the biocompatibility of polymeric materials was devised using the proliferation of BHK-21 Clone-13 cells. BHK cells cultured on a cover glass are brought in contact with the surface of a polymer and the growth of cells on the surface is examined. The method is simple and will be useful as an auxiliary to an implantation test. Growth rates and protein contents of cells were investigated for polyacrylates and segmented polyurethanes. Good proliferation was obtained on the surface of poly (methyl methacrylate) and an abnormal one on poly (2-hydroxyethyl methacrylate). For segmented polyurethanes, good proliferation was obtained on the polymers extended with ethylenediamine and poor proliferation on the polymers extended with hydrazine. © 1979, The Society of Polymer Science, Japan. All rights reserved.