REGULATION OF TRANSFORMING GROWTH FACTOR-ALPHA MESSENGER-RNA EXPRESSION IN T3M4 HUMAN PANCREATIC-CARCINOMA CELLS

被引：26

作者：

GLINSMANNGIBSON, BJ ^{[1
]}

KORC, M ^{[1
]}

机构：

[1] UNIV CALIF IRVINE,DEPT MED,DIV ENDOCRINOL & METAB,MED SCI 1,C240,IRVINE,CA 92717

来源：

PANCREAS | 1991年 / 6卷 / 02期

关键词：

TRANSFORMING GROWTH FACTOR-ALPHA; EPIDERMAL GROWTH FACTOR RECEPTOR; AUTOCRINE REGULATION; PANCREATIC CANCER;

D O I：

10.1097/00006676-199103000-00003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Cultured human pancreatic cancer cells produce transforming growth factor-alpha (TGF-alpha), a potent mitogenic polypeptide. In the present study, we investigated the regulation of TGF-alpha mRNA expression in T3M4 human pancreatic carcinoma cells. TGF-alpha mRNA levels were quantitated by densitometric analysis of autoradiographs obtained following hybridization of size-fractionated cytoplasmic RNA with P-32-labeled cRNA coding for human TGF-alpha. There was a twofold increase in TGF-alpha mRNA levels at 2 h following addition of either epidermal growth factor (EGF) or TGF-alpha. However, TGF-alpha mRNA levels declined to near basal levels by 10 h. At 2 h, one-half maximal stimulation of TGF-alpha mRNA levels occurred at 1 nM and maximal stimulation at 4 nM of either EGF or TGF-alpha. The transcriptional inhibitor actinomycin D (Act D) and the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), mimicked the actions of EGF and TGF-alpha. These findings indicate that the regulation of TGF-alpha mRNA expression in T3M4 cells is complex, and is mediated, in part, via the EGF receptor.

引用

页码：142 / 149

页数：8

共 43 条

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