THE TRICARBOXYLATE CARRIER FROM RAT-LIVER MITOCHONDRIA - PURIFICATION, RECONSTITUTION AND KINETIC CHARACTERIZATION

被引：20

作者：

GLERUM, DM ^{[1
]}

CLAEYS, D ^{[1
]}

MERTENS, W ^{[1
]}

AZZI, A ^{[1
]}

机构：

[1] UNIV BERN, INST BIOCHEM & MOLEK BIOL, BUHLSTR 28, CH-3012 BERN, SWITZERLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 194卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15668.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A K(m) of 40-mu-M and a V(max) of 1.56-mu-mol x min-1 mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.

引用

页码：681 / 684

页数：4

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