SYNAPTIC PATHOLOGY IN ALZHEIMERS-DISEASE - IMMUNOLOGICAL DATA FOR MARKERS OF SYNAPTIC AND LARGE DENSE-CORE VESICLES

被引:155
作者
LASSMANN, H
WEILER, R
FISCHER, P
BANCHER, C
JELLINGER, K
FLOOR, E
DANIELCZYK, W
SEITELBERGER, F
WINKLER, H
机构
[1] UNIV VIENNA, INST NEUROL, A-1010 VIENNA, AUSTRIA
[2] UNIV INNSBRUCK, DEPT PHARMACOL, A-6020 INNSBRUCK, AUSTRIA
[3] UNIV KANSAS, DEPT ANAT, LAWRENCE, KS 66045 USA
[4] LUDWIG BOLTZMANN INST AGEING RES, ALZHEIMER DEMENTIA SECT, VIENNA, AUSTRIA
[5] LUDWIG BOLTZMANN INST CLIN NEUROBIOL, VIENNA, AUSTRIA
关键词
D O I
10.1016/0306-4522(92)90003-K
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have analysed several markers for small synaptic vesicles (synaptin-synaptophysin, p65 and SV2) and large dense-core vesicles (chromogranin A, secretogranin II/chromogranin C) in the brains of patients with Alzheimer's disease, and normal controls by immunoblotting and immunohistochemistry. In comparison to age-matched controls the levels of all three synaptic vesicle markers were decreased in temporal cortex of Alzheimer patients. On the other hand, the levels of chromogranin A were increased, and those of secretogranin II lowered. This resulted in a significant increase of the ratios of chromogranin A to synaptophysin, p65 or SV2 and of that for chromogranin A to secretogranin II. These increases were significantly correlated to clinical severity of dementia and extent of neuropathological changes. By immunohistochemistry a high percentage of senile plaques was found to contain chromogranin A-reactive dystrophic neurites, whereas synaptophysin reactivity within plaques was rare. These results indicate that the number of synaptic vesicles is lowered in Alzheimer's disease, and that one component of large dense-core vesicles, i.e. chromogranin A, is elevated. We, thus, suggest that in Alzheimer's brain distinct changes occur for both types of synaptic organelles.
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页码:1 / 8
页数:8
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