IMPROVEMENTS IN THE METHOD DEVELOPED FOR PERFORMING ISOELECTRIC-FOCUSING IN UNCOATED CAPILLARIES

被引：41

作者：

MAZZEO, JR ^{[1
]}

KRULL, IS ^{[1
]}

机构：

[1] NORTHEASTERN UNIV,BARNETT INST,DEPT CHEM,341 MU,BOSTON,MA 02115

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 606卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)87041-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary isoelectric focusing run times were reduced from 30 to 5 minutes through reversing the polarity and shortening the separation distance. Complete resolution was only obtained by increasing the concentration of tetramethylethylenediamine in the sample solution. Problems previously seen with acidic proteins were shown to be due to anodic drift in the ampholyte gradient, and were minimized by increasing the concentration of phosphoric acid in the anode buffer. The inability of the method to tolerate protein samples with salt is also discussed.

引用

页码：291 / 296

页数：6

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