REPLICATION OF PLASMID-DERIVED HUMAN PAPILLOMAVIRUS TYPE-11 DNA IN CULTURED KERATINOCYTES

被引：36

作者：

MUNGAL, S

STEINBERG, BM

TAICHMAN, LB

机构：

[1] SUNY STONY BROOK, SCH DENT MED, DEPT ORAL BIOL & PATHOL, STONY BROOK, NY 11794 USA

[2] LONG ISL JEWISH MED CTR, DEPT OTOLARYNGOL, NEW HYDE PK, NY 11042 USA

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 05期

关键词：

D O I：

10.1128/JVI.66.5.3220-3224.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmid-derived human papillomavirus type 11 (HPV11) DNA has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. HPV11 DNA was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with pSV2Neo. Transfectants were selected and shown to contain replicated episomal HPV DNA. Once selected, HPV11 DNA persists in cells through at least two additional passages.

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页码：3220 / 3224

页数：5

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