IMMUNOHISTOCHEMICAL LOCALIZATION OF L-TYPE CALCIUM CHANNELS IN THE DEVELOPING 1ST MOLAR OF THE RAT DURING ODONTOBLAST DIFFERENTIATION

To study the presence of L-type calcium channels during the different steps of odontoblast differentiation, a specific monoclonal antibody against 1,4-dihydropyridine receptors was used in combination with avidin-biotin-peroxidase complex labelling. Staining was seen on the cell bodies of pre-odontoblasts, concentrated at the apical pole (distal portion) of functional odontoblasts and localized on cell bodies and processes of mature odontoblasts. Thus calcium channels were expressed at the onset of differentiation and maintained in the differentiated cells but with some changes of localization. It is suggested that these channels may facilitate the entry of calcium to act as a second messenger for cellular polarization or be involved in the transcellular transport of calcium to the mineralizing front.