A CLOSTRIDIUM-PERFRINGENS VECTOR FOR THE SELECTION OF PROMOTERS

被引：21

作者：

MATSUSHITA, C ^{[1
]}

MATSUSHITA, O ^{[1
]}

KOYAMA, M ^{[1
]}

OKABE, A ^{[1
]}

机构：

[1] KAGAWA MED SCH,DEPT MICROBIOL,KAGAWA 76107,JAPAN

来源：

PLASMID | 1994年 / 31卷 / 03期

关键词：

D O I：

10.1006/plas.1994.1035

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A promoter selection vector for Clostridium perfringens genes was constructed from a C. perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from plP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be a useful reporter system for C. perfringens genes. (C) 1994 Academic Press, Inc.

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收藏

页码：317 / 319

页数：3

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