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THE ROLE OF THE 9TH AND 10TH TYPE-III DOMAINS OF HUMAN FIBRONECTIN IN CELL-ADHESION

被引：80
作者：
MARDON, HJ
GRANT, KE
机构：
[1] Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, 0X3 9DU, Headington
来源：
FEBS LETTERS | 1994年 / 340卷 / 03期
关键词：
FIBRONECTIN; FIII DOMAIN; RGD; CELL ADHESION; CELL SPREADING ASSAY; PROTEIN EXPRESSION;
D O I：
10.1016/0014-5793(94)80137-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fibronectins (FN) contain sites, in addition to the cell recognition site RGD in the tenth type III domain (FIII10), that are required for adhesive activity. The role of FIII10 and the adjacent FIII9 was analysed in functional cell adhesion assays recombinant FIII domains in which the domain boundaries were strictly conserved. FIII9 had no adhesive activity. FIII10, and FIII9 plus FIII10 had less activity than FN, whereas the activity of FIII9-10 was similar to FN. We conclude that FIII9 acts synergistically with FIII10 in cell adhesion, and that this synergy is dependent upon the structural integrity of the FIII9-10 pair of domains.
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页码：197 / 201
页数：5
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