LYSIS OF GLYCOSIDES IN PRESENCE OF L-ASCORBIC ACID

被引:8
作者
CAYGILL, JC
机构
[1] Rheumatism Research Centre, University of Manchester, Manchester, 13, Clinical Sciences Building
关键词
D O I
10.1016/0304-4165(68)90155-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The liberation of nitrophenol from eleven nitrophenyl glycosides in acid or neutral solution in the presence of l-ascorbic acid is reported. The conditions under which o-nitrophenol β-d-galactopyranoside are reported. The reaction rate, which is proportional to temperature and glycoside concentration, is maximal between pH 4 and 5, but negligible above pH 8.5, and is increased by Cu2+. At low concentrations of l-ascorbic acid the reaction rate is proportional to l-ascorbic acid concentration, but at higher concentrations the reaction rate is not further increased. Other monosaccharides inhibit the release of nitrophenol. © 1968.
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