HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC SEPARATION OF 2 MAJOR TOXIC COMPOUNDS FROM GYMNODINIUM-BREVE DAVIS

Cultured Gymnodinium breve cells were extracted in acidified ether and fractionated by a new, convenient procedure utilizing thin layer chromatography or elution dry column chromatography. The most toxic fraction was further separated either directly by analytical high pressure liquid chromatography (HPLC) or in subsequent work by preparative, followed by analytical HPLC. Two toxic compounds, designated T4g and T47, were isolated; purity of each was demonstrated by rechromatography in analytical HPLC with both adsorptive and reverse phase packings. Both the single pass and recycle modes of operation were used with two detector systems in each to demonstrate a single entity. UV, fluorescence, and stability data differentiated these compounds from previously described, less toxic components from 6. breve. © 1979 Oxford University Press.