SCANNING ELECTRON-MICROSCOPY OF FROZEN-SECTIONS - METHOD FOR STUDYING SELECTED PLANES IN SMALL SPECIMENS

被引：2

作者：

CARRI, N ^{[1
]}

SUBURO, A ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC MED,INST BIOL CELLULAIRE,BUENOS AIRES,ARGENTINA

来源：

ANATOMICAL RECORD | 1979年 / 193卷 / 04期

关键词：

D O I：

10.1002/ar.1091930408

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

A procedure for studying small biological specimens with the SEM, using frozen sections of fixed material is described. Controlled fixation schedules and convenient handling of sections insure optimal preservation of cell shape and size. The greatest advantage of the method presented is to allow scanning through any desired plane of the specimen, even in those planes parallel to an epithelial face. SEM of frozen sections is thus helpful for the study of cell distribution and organization within a tissue. Copyright © 1979 Wiley‐Liss, Inc.

引用

页码：857 / 861

页数：5

共 10 条

[1] SCANNING ELECTRON MICROSCOPY OF CHICK-EMBRYO [J].

ARMSTRON.PB ;

PARENTI, D .

DEVELOPMENTAL BIOLOGY, 1973, 33 (02) :457-462

[2] Preparation of animal tissues for surface-scanning electron microscopy [J].

Boyde, A. ;

Wood, C. .

JOURNAL OF MICROSCOPY, 1969, 90 :221-249

[3] A SERIES OF NORMAL STAGES IN THE DEVELOPMENT OF THE CHICK EMBRYO [J].

HAMBURGER, V ;

HAMILTON, HL .

JOURNAL OF MORPHOLOGY, 1951, 88 (01) :49-&

[4]

Humphreys WJ, 1974, SCANNING ELECTRON MI, P275

[5]

MESTRES P, 1977, SCANNING ELECTRON MI, V2, P381

[6]

SCOTT J. E., 1960, METHODS BIOCHEM ANAL, V8, P145

[7] SCANNING AND TRANSMISSION ELECTRON-MICROSCOPE STUDIES OF INTERKINETIC NUCLEAR MIGRATION IN CEREBRAL VESICLES OF RAT [J].

SEYMOUR, RM ;

BERRY, M .

JOURNAL OF COMPARATIVE NEUROLOGY, 1975, 160 (01) :105-125

[8] ENVIRONMENT OF AXONAL MIGRATION IN THE DEVELOPING CHICK RETINA - SCANNING ELECTRON-MICROSCOPIC (SEM) STUDY [J].

SUBURO, A ;

CARRI, N ;

ADLER, R .

JOURNAL OF COMPARATIVE NEUROLOGY, 1979, 184 (03) :519-+

[9] SCANNING ELECTRON-MICROSCOPIC COMPARISON OF DEVELOPMENT OF EMBRYONIC AND REGENERATING LIMBS IN AXOLOTL [J].

TANK, PW ;

CARLSON, BM ;

CONNELLY, TG .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1977, 201 (03) :417-429

[10] SCANNING MICROSCOPY OF DISSOCIATED TISSUE CELLS [J].

VIAL, J ;

PORTER, KR .

JOURNAL OF CELL BIOLOGY, 1975, 67 (02) :345-360

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