MIGRATION ASSAY FOR ENDOTHELIAL-CELLS IN MULTIWELLS - APPLICATION TO STUDIES ON THE EFFECT OF OPIOIDS

被引：41

作者：

FISCHER, EG

STINGL, A

KIRKPATRICK, CJ

机构：

[1] Institute of Pathology, RWTH-Klinikum

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 128卷 / 02期

关键词：

Cell migration; Cell proliferation; Endothelial cell; Endothelial gwoth factor; β-Endorphin;

D O I：

10.1016/0022-1759(90)90215-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay system is described which permits rapid and effective evaluation of endothelial cell repair, using cells growing in a monolayer. With this method it was possible to obtain highly significant results. For example, endothelial growth factor and heparin, significantly enhanced cell migration and/or proliferation, whereas β-endorphin, an endogenous opioid, had no effect on the migration and/or proliferation of human umblical vein endothelial cells. This model may be used to study the cell migration of a variety of cell types which under certain experimental conditions (e.g., irradiation) do not proliferate. © 1990.

引用

页码：235 / 239

页数：5

共 14 条

[1]

BOYDEN S, 1962, J EXP MED, V115, P543

[2] CELL-SHAPE OF POLYMORPHONUCLEAR LEUKOCYTES IS INFLUENCED BY OPIOIDS [J].