THE ANALYSIS OF UNDERIVATISED OLIGOSACCHARIDES BY MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY

被引：136

作者：

MOCK, KK

DAVEY, M

COTTRELL, JS

机构：

[1] Finnigan MAT Ltd., Paradise, Hemel Hempstead

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91837-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix Assisted Laser Desorption Mass Spectrometry is shown to provide a rapid and sensitive technique for the analysis of underivatised oligosaccharides. Typical sample loading is 1 pmol and analysis time is around 5 minutes. Through the use of an internal standard, mass measurements are generally accurate to within 0.5 Da. The technique is particularly useful for the analysis of oligosaccharide mixtures released from glycoproteins. © 1991.

引用

页码：644 / 651

页数：8

共 22 条

[1] Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P432, DOI 10.1002/rcm.1290031207
[2] Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P233, DOI 10.1002/rcm.1290030708
[3] RAPID, SENSITIVE ANALYSIS OF PROTEIN MIXTURES BY MASS-SPECTROMETRY
BEAVIS, RC
CHAIT, BT
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) : 6873 - 6877
[4] CAPRIOLI RM, 1991, TECHNIQUES PROTEIN C, V2
[5] STRUCTURAL FINGERPRINTING OF ASN-LINKED CARBOHYDRATES FROM SPECIFIC ATTACHMENT SITES IN GLYCOPROTEINS BY MASS-SPECTROMETRY - APPLICATION TO TISSUE PLASMINOGEN-ACTIVATOR
CARR, SA
ROBERTS, GD
JUREWICZ, A
FREDERICK, B
[J]. BIOCHIMIE, 1988, 70 (11) : 1445 - 1454
[6] CARBOHYDRATE MAPPING BY MASS-SPECTROMETRY - A NOVEL METHOD FOR IDENTIFYING ATTACHMENT SITES OF ASN-LINKED SUGARS IN GLYCOPROTEINS
CARR, SA
ROBERTS, GD
[J]. ANALYTICAL BIOCHEMISTRY, 1986, 157 (02) : 396 - 406
[7] FAB-MASS SPECTROMETRY OF CARBOHYDRATES
DELL, A
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1987, 45 : 19 - 72
[8] EGGE H, 1991, IN PRESS PURE APPL C
[9] PROPERTIES OF MATRIX-ASSISTED LASER DESORPTION - MEASUREMENTS WITH A TIME-TO-DIGITAL CONVERTER
ENS, W
MAO, Y
MAYER, F
STANDING, KG
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1991, 5 (03) : 117 - 123
[10] GILLECECASTRO BL, 1990, BIOLOGICAL MASS SPECTROMETRY /, P411

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