CHONDRONS FROM ARTICULAR-CARTILAGE .3. MORPHOLOGICAL-CHANGES IN THE CELLULAR MICROENVIRONMENT OF CHONDRONS ISOLATED FROM OSTEOARTHRITIC CARTILAGE

被引：93

作者：

POOLE, CA ^{[1
]}

MATSUOKA, A ^{[1
]}

SCHOFIELD, JR ^{[1
]}

机构：

[1] YAMAGUCHI UNIV, SCH MED, DEPT ORTHOPAED SURG, UBE, YAMAGUCHI 755, JAPAN

来源：

ARTHRITIS AND RHEUMATISM | 1991年 / 34卷 / 01期

关键词：

D O I：

10.1002/art.1780340105

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Chondrons were isolated from human and canine osteoarthritic cartilage using low-speed homogenization techniques. Changes in chondron morphology were evaluated using differential interference-contrast microscopy, phase-contrast microscopy, and histochemical and ultrastructural methods. Chondrocyte viability was assessed using fluorescein diacetate staining, and chondron metabolism was investigated using autoradiography. The results suggest that initial changes in the collagen and proteoglycan distribution within the chondron are followed by chondrocyte proliferation to form clusters. These techniques offer the potential to study cell matrix interactions in degenerative osteoarthritis.

引用

页码：22 / 35

页数：14

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