AFFINITY ELECTROPHORESIS USED FOR DETERMINATION OF BINDING CONSTANTS FOR ANTIBODY ANTIGEN REACTIONS

被引：17

作者：

HEEGAARD, NHH

BJERRUM, OJ

机构：

[1] NIMH,NEUROSCI CTR ST ELIZABETHS,BIOCHEM GENET LAB,WASHINGTON,DC 20032

[2] NOVO NORDISK,DK-2880 BAGSVAERD,DENMARK

[3] UNIV COPENHAGEN,PROT LAB,DK-2200 COPENHAGEN,DENMARK

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90337-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of affinity electrophoresis in agarose gels for determination of binding constants for the interaction of antigens with monoclonal antibodies is exemplified for monoclonal anti-human serum albumin and anti-α1-fetoprotein antibodies. The calculated binding constants are verified by independent binding assays. The electrophoretic separation of antigen-antibody complexes of different stoichiometry is also demonstrated. Thus, affinity electrophoresis represents an alternative method for both qualitative and quantitative assessment of antigen-antibody interactions. © 1991.

引用

页码：319 / 326

页数：8

共 28 条

[1]

AXELSEN NH, 1983, SCAND J IMMUNOL S10, V17

[2] MEASUREMENT OF ANTIBODY-AFFINITY FOR CELL-SURFACE ANTIGENS USING AN ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].