A LARGE UNINTEGRATED RETROVIRUS DNA SPECIES PRESENT IN A DERMAL TUMOR OF WALLEYE STIZOSTEDION-VITREUM

被引：51

作者：

MARTINEAU, D ^{[1
]}

RENSHAW, R ^{[1
]}

WILLIAMS, JR ^{[1
]}

CASEY, JW ^{[1
]}

BOWSER, PR ^{[1
]}

机构：

[1] CORNELL UNIV,NEW YORK STATE COLL VET MED,DEPT AVIA & AGUAT ANIM MED,ITHACA,NY 14853

来源：

DISEASES OF AQUATIC ORGANISMS | 1991年 / 10卷 / 03期

关键词：

D O I：

10.3354/dao010153

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Throughout North America, up to 27% of adult walleye (Pisces: Stizostedion vitreum) are seasonally affected by a dermal tumor termed walleye dermal sarcoma (WDS) which is associated with C-type retrovirus-like particles. A homogenate from 20 pooled tumors was fractionated by sucrose density gradient centrifugation and retrovirus particles were identified by electron microscopy in fractions with high reverse transcriptase (RT) activity. RNA was isolated from a sucrose density gradient fraction which contained a major portion of the RT activity and which had a density of 1.18 g ml-1. Denaturing gel electrophoresis showed the presence of a minor 12 kb RNA species, presumed to represent the undegraded genomic RNA of walleye dermal sarcoma virus (WDSV). Digoxigenin-labeled cDNA synthesized from this viral RNA preparation specifically hybridized with a 13 kb linear unintegrated viral DNA species present only in DNA from walleye tumors. These findings strongly suggest that WDS is the result of an infection caused by a unique exogenous retrovirus of which the unusually large genome is predominantly unintegrated in tumor cells.

引用

页码：153 / 158

页数：6