CHARACTERIZATION OF A DIMERIC CANINE FORM OF SURFACTANT PROTEIN C(SP-C)

被引:28
作者
CREUWELS, LAJM
DEMEL, RA
VANGOLDE, LMG
HAAGSMAN, HP
机构
[1] UNIV UTRECHT,VET BIOCHEM LAB,3508 TD UTRECHT,NETHERLANDS
[2] UNIV UTRECHT,CTR BIOMEMBRANES & LIPID ENZYMOL,3508 TD UTRECHT,NETHERLANDS
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1254卷 / 03期
关键词
HYDROPHOBIC SURFACTANT PROTEIN; ACYLATED PROTEIN; DIMERIC PROTEIN; SURFACE TENSION; PULMONARY SURFACTANT;
D O I
10.1016/0005-2760(94)00195-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Canine pulmonary surfactant protein C (SP-C) is a small hydrophobic peptide which has one palmitoylated cysteine residue. SP-C enhances the insertion of phospholipids into a monolayer. Two forms of canine SP-C were isolated using Sephadex LH-60 chromatography. It was found that canine SP-C exists in a palmitoylated monomeric form of 3.5 kDa, and a non-acylated dimeric form of 7 kDa. Circular dichroism showed that both forms of SP-C exhibit similar secondary structures at the air/water interface. Both forms of SP-C were able to induce the insertion of phospholipids into a monolayer as measured with the Wilhelmy plate technique. In contrast to the palmitoylated monomeric form of SP-C, the non-acylated dimeric form of SP-C does not require calcium ions to insert phospholipids into a monolayer without the negatively charged phosphatidylglycerol. It is concluded that two forms of canine SP-C exist, but the physiological significance of these different forms remains to be established.
引用
收藏
页码:326 / 332
页数:7
相关论文
共 30 条
[1]   VAPOR-PHASE MODIFICATION OF SULFHYDRYL-GROUPS IN PROTEINS [J].
AMONS, R .
FEBS LETTERS, 1987, 212 (01) :68-72
[2]   STRUCTURE AND FUNCTIONS OF A DIMERIC FORM OF SURFACTANT PROTEIN SP-C - A FOURIER-TRANSFORM INFRARED AND SURFACTOMETRY STUDY [J].
BAATZ, JE ;
SMYTH, KL ;
WHITSETT, JA ;
BAXTER, C ;
ABSOLOM, DR .
CHEMISTRY AND PHYSICS OF LIPIDS, 1992, 63 (1-2) :91-104
[3]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[4]   EFFECT OF SURFACTANT-ASSOCIATED PROTEIN-A (SP-A) ON THE ACTIVITY OF LIPID EXTRACT SURFACTANT [J].
CHUNG, J ;
YU, SH ;
WHITSETT, JA ;
HARDING, PGR ;
POSSMAYER, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1002 (03) :348-358
[5]  
CREUWELS LAJM, 1993, J BIOL CHEM, V268, P26752
[6]   HYDROPHOBIC SURFACTANT-ASSOCIATED POLYPEPTIDES - SP-C IS A LIPOPEPTIDE WITH 2 PALMITOYLATED CYSTEINE RESIDUES, WHEREAS SP-B LACKS COVALENTLY LINKED FATTY ACYL-GROUPS [J].
CURSTEDT, T ;
JOHANSSON, J ;
PERSSON, P ;
EKLUND, A ;
ROBERTSON, B ;
LOWENADLER, B ;
JORNVALL, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :2985-2989
[7]  
GLASSER SW, 1988, J BIOL CHEM, V263, P9
[8]  
HAAGSMAN HP, 1987, J BIOL CHEM, V262, P13877
[9]   SYNTHESIS AND ASSEMBLY OF LUNG SURFACTANT [J].
HAAGSMAN, HP ;
VANGOLDE, LMG .
ANNUAL REVIEW OF PHYSIOLOGY, 1991, 53 :441-464
[10]   NUCLEOTIDE AND AMINO-ACID-SEQUENCES OF PULMONARY SURFACTANT PROTEIN SP-18 AND EVIDENCE FOR COOPERATION BETWEEN SP-18 AND SP 28-36 IN SURFACTANT LIPID ADSORPTION [J].
HAWGOOD, S ;
BENSON, BJ ;
SCHILLING, J ;
DAMM, D ;
CLEMENTS, JA ;
WHITE, RT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (01) :66-70