THE CAPSICUM L(3) GENE-MEDIATED RESISTANCE AGAINST THE TOBAMOVIRUSES IS ELICITED BY THE COAT PROTEIN

被引：129

作者：

BERZALHERRANZ, A ^{[1
]}

DELACRUZ, A ^{[1
]}

TENLLADO, F ^{[1
]}

DIAZRUIZ, JR ^{[1
]}

LOPEZ, L ^{[1
]}

SANZ, AI ^{[1
]}

VAQUERO, C ^{[1
]}

SERRA, MT ^{[1
]}

GARCIALUQUE, I ^{[1
]}

机构：

[1] CSIC,CTR INVEST BIOL,DEPT BIOL PLANTAS,E-28006 MADRID,SPAIN

来源：

VIROLOGY | 1995年 / 209卷 / 02期

关键词：

D O I：

10.1006/viro.1995.1282

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The L(3) gene is responsible for the hypersensitive response in Capsicum plants against infection by tobamoviruses. The resistance conferred by this gene is one of the most effective so far described against tobamoviruses. Certain isolates of pepper mild mottle virus (PMMV) are the only tobamoviruses able to overcome the L(3) resistance. Chimeric viral genomes between PMMV-S (to which L(3) plants are hypersensitive) and PMMV-I (an L(3) resistance-breaking isolate) led us to conclude that sequence variation within the coal protein gene of both isolates determines their different virulence in L(3)L(3) plants. Furthermore, the results indicate that a single amino acid substitution, Asn to Met, at position 138 of the PMMV-I coat protein is sufficient to induce the hypersensitive response and localization of viral infection in C. chinense plants. Finally, the use of a mutant coding for a truncated coat protein (maintaining the Met(138), coding sequence at the RNA level) demonstrates that a functional coat protein is required for elicitation of the L(3) gene-mediated resistance. (C) 1995 Academic Press, Inc.

引用

页码：498 / 505

页数：8

共 32 条

[1] Alonso E., Garcia-Luque L., Avila-Rincon M.J., Wicke B., Serra M.T., Diaz-Rulz J.R., A tobamovlrus causing heavy losses in protected pepper crops in Spain, J, PhytoPathol, 125, pp. 67-76, (1989)
[2] Alonso E., Garcia-Luque I., De La Cruz A., Wicke B., Avila-Rincon M.J., Serra M.T., Castresana C., Diaz-Ruiz J.R., Nucleotide sequence of the genomic RINA of pepper mild mottle virus, a resistance-breaking tobamovirus in pepper, J. Gen. Virol, 72, pp. 2875-2884, (1991)
[3] Avrameas S., Coupling of enzymes to proteins with glutaralde-hydc. Use of the conjugates for the detection of antigens and antibodies, Immunochemistry, 6, pp. 43-53, (1969)
[4] Beck D.L., Forster R.L.S., Bevan M.W., Boxen K.A., Lowe S.C., Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus, Virology, 177, pp. 152-158, (1990)
[5] Bloomer A.C., Champness J.N., Bricogne C., Staden R., Klug A., Protein disk of tobacco mosaic virus at 2.8 Å resolution showing the interactions within and between subunits, Nature, 276, pp. 362-368, (1978)
[6] Boukema I.W., Resistance to TMV in Capsicum chacoense Hunz. Is governed by an allele of the L-locus, Capsicum News!, 3, pp. 47-48, (1982)
[7] Boukema I.W., Jansen K., Hofman K., Strains of TMV and Genes for Resistance in Capsicum, pp. 44-48, (1980)
[8] Clark M.F., Adams A.N., Characteristics of a microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses, J. Gen. Virol, 34, pp. 475-483, (1977)
[9] Culver J.N., Dawson W.O., Tobacco mosaic virus elicitor coat protein genes produce a hypersensitive phenotype in transgenic Nicotiana sylvestris plants, Mol. Plant-Microbe Interact, 4, pp. 458-463, (1991)
[10] Culver J.N., Lindbeck A.G.C., Dawson W.O., Virus-host interactions: Induction of chlorotic and necrotic responses in plants by tobamoviruses, Annu. Rev. Phytopathoi, 29, pp. 193-217, (1991)

← 1 2 3 4 →