CCD CAMERA IMAGING FOR THE CHEMILUMINESCENT DETECTION OF ENZYMES USING NEW ULTRASENSITIVE REAGENTS

We have designed and constructed an inexpensive imaging system based on charge-coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen(R) chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi-Phos(R) Plus and Lumigen PS. Lumi-Phos Plus is an enhanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme-linked immunoassays and nucleic acid probe assays using HRP conjugates.