TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAYS FOR URINARY LUTEINIZING-HORMONE AND FOLLICLE-STIMULATING-HORMONE

被引：24

作者：

KESNER, JS

KNECHT, EA

KRIEG, EF

机构：

[1] Experimental Toxicology Branch and Statistics Activity, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, Cincinnati, OH 45226-1998, 4676 Columbia Parkway

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 285卷 / 1-2期

关键词：

FLUOROMETRY; EPIDEMIOLOGY; GONADOTROPINS; MENSTRUAL CYCLE; 2-SITE IMMUNOFLUOROMETRIC ASSAY;

D O I：

10.1016/0003-2670(94)85003-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The goal of this effort was to develop and validate non-radioisotopic immunoassays for measuring luteinizing hormone and follicle stimulating hormone in unextracted urine. Towards this goal, commercial time-resolved immunofluorometric assays (IFMAs) were modified. Validation demonstrated that the resultant assays were specific, sensitive, accurate, and precise. Urine matrix was shown not to interfere with the assay. Gonadotropin profiles generated using these assays conformed to those measured in urine and serum by other established immunoassays. These IFMAs afford the collective advantages of non-radioisotopic procedures and urine sample collection (convenience, noninvasiveness, integration of pulsatile secretion), plus the superior sensitivity and specificity of IFMAs. Applications include epidemiology and medicine.

引用

页码：13 / 22

页数：10

共 32 条

[1] HORMONAL PROFILES OF NATURAL CONCEPTION CYCLES ENDING IN EARLY, UNRECOGNIZED PREGNANCY LOSS [J].