DIFFERENCES BETWEEN HUMAN HYBRIDOMA PLATELET-BINDING ANTIBODIES DERIVED FROM SYSTEMIC LUPUS-ERYTHEMATOSUS PATIENTS AND NORMAL INDIVIDUALS

被引：11

作者：

MENG, QH ^{[1
]}

RAUCH, J ^{[1
]}

机构：

[1] MCGILL UNIV,MONTREAL GEN HOSP,RES INST,RHEUMAT DIS UNIT,1650 CEDAR AVE,MONTREAL H3G 1A4,QUEBEC,CANADA

来源：

AUTOIMMUNITY | 1990年 / 5卷 / 03期

基金：

英国医学研究理事会;

关键词：

Anti-phospholipid; Hybridoma autoantibodies; Normal; Platelet-binding; Polyspecificity; SLE;

D O I：

10.3109/08916939009002974

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The binding and functional activities of platelet-binding hybridoma autoantibodies from SLE patients were compared with those derived from normal individuals. Twenty-nine SLE-derived hybridoma antibodies and 20 normal-derived hybridoma antibodies were analyzed for binding to glutaraldehyde fixed platelets, dDNA and phospholipids, and for lupus anticoagulant activity. Twenty-four of the 29 SLE-derived antibodies and 9 of the 20 normal-derived antibodies showed one or more activities in these assays. Of the 24 SLE-derived antibodies, 8 (33.3% were reactive in only one assay (monospecific), while the other 16 (66.7% had more than one of these activities (polyspecific). In contrast, none (0% of the 9 normal-derived antibodies with known activities were monospecific, while all 9 (100% showed polyspecificity. Statistical analyses demonstrated that there was no correlation of anti-DNA activity with anti-platelet and most anti-phospholipid activities for the SLE-derived antibodies, and strong positive correlations between these reactivities for the normal-derived antibodies. Similarly, differences were observed in Western blotting analyses; SLE-derived antibodies bound more specifically to individual platelet proteins than normal-derived antibodies. Moreover, in chromium-51 release assays, all of the SLE-derived platelet-binding antibodies were cytotoxic to platelets, while none of the normal-derived platelet-binding antibodies showed significant cytotoxicity. Our results suggest that hybridoma platelet-binding autoantibodies derived from SLE patients exhibit greater antigen specificity and functional activity than similar antibodies derived from normal individuals. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：151 / 167

页数：17

共 33 条

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