THE C-KIT MOLECULE AND THE SURFACE IMMUNOPHENOTYPE OF HUMAN ACUTE-LEUKEMIA

被引：19

作者：

KUBOTA, A ^{[1
]}

OKAMURA, S ^{[1
]}

SHIMODA, K ^{[1
]}

HARADA, M ^{[1
]}

NIHO, Y ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT INTERNAL MED 1,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 14卷 / 5-6期

关键词：

C-KIT; IMMUNOPHENOTYPE; BIPHENOTYPIC LEUKEMIA; FLOW CYTOMETRY; STEM CELL FACTOR;

D O I：

10.3109/10428199409049699

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The proto-oncogene c-kit encodes the receptor for a stem cell factor (c-kit molecule). Expression of the c-kit molecule on the gated leukemic blast cells from newly diagnosed patients with leukemia was analysed by flow cytometry using the monoclonal antibody (17F11). Among 35 myeloid leukemia cases examined, significant c-kit-positive blast cells were detected in 24 cases (69%), even though the percentage of positive cells was widely variable. The correlation between the percentage of cells positive for the c-kit molecule and the percentage of cells positive for CD34 was found to be statistically significant (rs = 0.36, p < 0.05). Fifteen cases of myeloid leukemia were positive for lymphoid markers. The mean percentage of the cells expressing c-kit molecule among the lymphoid marker-positive cases was significantly larger than that among the lymphoid marker-negative cases (p < 0.05). All 19 lymphoid leukemia cases were c-kit-negative, including 8 cases which were positive for some myeloid markers. Stem cell factor enhanced the colony growth in five out of six acute myeloblastic leukemia cases expressing the c-kit molecule. On the other hand, SCF did not stimulate colony growth in any of the four cases which were not positive for the c-kit molecule. These findings indicated that the distribution of flow cytometrically detectable c-kit molecules on leukemic cells is related to the morphologic and immunologic classification of these leukemic cells and to the expression of the CD34 cell surface molecule on some myeloid leukemic cells. On such cells, expression of the c-kit molecule may have a functional role and be related to the maturation process.

引用

页码：421 / 428

页数：8

共 32 条

[1]

Yarden Y., Kuang W.J., Yang-Feng T., Coussens L., Munemitsu S., Dull T.J., Chen E., Schlessingfer J., Francke U., Ullrich A., Human proto-oncogene c-kit: A new cell surface receptor tyrosine kinase for an unidentified ligand, EMBO J., 6, pp. 3341-3351, (1987)

[2]

Qiu F.H., Ray P., Brown K., Barker P.E., Jhanwar S., Ruddle F.H., Bessmer P., Primary structure of c-kit: Relationship with the CSF-1/PDGF receptor kinase family-oncogenic activation of v-kit involves deletion of extracellular domain and C terminus, EMBO J., 7, pp. 1003-1011, (1988)

[3]

Keshet E., Lyman S.D., Williams D.E., Anderson D.M., Jenkins N.A., Copeland N.G., Parada L.F., Embryonic RNA expression patterns of the c-kit receptor and its cognate ligand suggest multiple functional roles in mouse development, EMBO J., 10, pp. 2425-2435, (1991)

[4]

Zsebo K.M., Williams D.A., Geissler E.N., Broudy V.C., Martin F.H., Atkins H.L., Hsu R., Birkett N.C., Okino K.H., Murdock D.C., Jacobsen F.W., Langley K.E., Smith K.A., Takeishi T., Cattanach B.M., Galli S.J., Suggs S.V., Stem cell factor is encoded at the S1 locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor, Cell, 63, pp. 213-224, (1990)

[5]

Copeland N.G., Gilbert D.J., Cho B.C., Donovan P.J., Jenkins N.A., Cosman D., Anderson D., Lyman S.D., Williams D.E., Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles, Cell, 63, pp. 175-183, (1990)

[6]

Williams D.E., Eisenman J., Baird A., Rauch C., van Ness K., March C.J., Park L.S., Martin U., Mochizuki D.Y., Boswell H.S., Burgess G.S., Cosman D., Lyman S.D., Identification of a ligand for the c-kit proto-oncogene, Cell, 63, pp. 167-174, (1990)

[7]

Huang E., Nocka K., Beier D.R., Chu T.Y., Buck J., Lahm H.W., Wellner D., Leder P., Besmer P., The hematopoietic growth factor KL is encoded by the SI locus and is the ligand of the c-kit receptor, the gene product of the W locus, Cell, 63, pp. 225-233, (1990)

[8]

Broxmeyer H.E., Cooper S., Lu L., Hangoc G., Anderson D., Cosman D., Lyman S.D., Williams D.E., Effect of murine mast cell growth factor (c-kit proto-oncogene ligand) on colony formation by human bone marrow hematopoietic progenitor cells, Blood, 77, pp. 2142-2149, (1991)

[9]

Wang C., Curtis J.E., Geissler E.N., McCulloch E.A., Minden M.D., The expression of the proto-on-cogene c-kit in the blast cells of acute myeloblasts leukemia, Leukemia, 3, pp. 699-702, (1989)

[10]

Ikeda H., Kanakura Y., Tamaki T., Kuriu A., Kitayama H., Ishikawa J., Kanayama Y., Yonezawa T., Tarui S., Griffin J.D., Expression and functional role of the proto-oncogene c-kit in acute myeloblasts leukemia cells, Blood, 78, pp. 2962-2968, (1991)

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