BRAIN-TYPE N-GLYCOSYLATION OF ASIALO-TRANSFERRIN FROM HUMAN CEREBROSPINAL-FLUID

被引：76

作者：

HOFFMANN, A

NIMTZ, M

GETZLAFF, R

CONRADT, HS

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,DEPT MOLEC BIOL,D-38124 BRAUNSCHWEIG,GERMANY

[2] GESELL BIOTECHNOL FORSCH MBH,DEPT MOLEC & INSTRUMENTAL STRUCT RES,D-38124 BRAUNSCHWEIG,GERMANY

来源：

FEBS LETTERS | 1995年 / 359卷 / 2-3期

关键词：

N-GLYCOSYLATION (BRAIN-TYPE); CEREBROSPINAL FLUID; HUMAN TRANSFERRIN; HUMAN BETA-TRACE PROTEIN;

D O I：

10.1016/0014-5793(95)00034-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Asialo-transferrin from human cerebrospinal fluid was purified to homogeneity, Investigation of the structural characteristics of its oligosaccharides support our hypothesis of 'brain-type' glycosylation of intrathecally synthesized cerebrospinal fluid proteins, For carbohydrate structural analysis, high-pH anion-exchange chromatography, methylation analysis, liquid secondary ion- and matrix-assisted laser desorption/ ionization mass spectrometry of the permethylated derivatives were used, The major structure turned out to be a complex-type agalactodiantennary oligosaccharide with bisecting N-acetylglucosamine and proximal fucose, Analysis of a second transferrin preparation containing both asialo- and sialo-transferrin revealed another major glycan species derived from the sialylated transferrin variant which is galactosylated and lacks bisecting N-acetylglucosamine and fucose.

引用

页码：164 / 168

页数：5

共 30 条

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