COMPLETE SEQUENCE OF THE EXTRACHROMOSOMAL RDNA MOLECULE FROM THE CILIATE TETRAHYMENA-THERMOPHILA STRAIN B1868VII

被引：59

作者：

ENGBERG, J

NIELSEN, H

机构：

[1] Department of Biochemistry B, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 23期

关键词：

D O I：

10.1093/nar/18.23.6915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.

引用

页码：6915 / 6919

页数：5

共 35 条

[1]

Blackburn E.H., 1986, P155

[2] A HIGH-AFFINITY TOPOISOMERASE-1 BINDING SEQUENCE IS CLUSTERED AT DNAASE-1 HYPERSENSITIVE SITES IN TETRAHYMENA R-CHROMATIN [J].