ELECTROPHORETIC SEPARATION OF GAMMA-A AND GAMMA-G HUMAN GLOBIN CHAINS IN NONIDET P-40

被引：16

作者：

GUERRASIO, A

SAGLIO, G

MAZZA, U

PICH, P

CAMASCHELLA, C

RICCO, G

GIANAZZA, E

RIGHETTI, PG

机构：

[1] UNIV MILAN,DEPT BIOCHEM,I-20133 MILAN,ITALY

[2] UNIV TURIN,MED 2 CLIN,IST MED INTERNA,I-10126 TURIN,ITALY

来源：

CLINICA CHIMICA ACTA | 1979年 / 99卷 / 01期

关键词：

D O I：

10.1016/0009-8981(79)90133-5

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, Aγ and Gγ human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment 7CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the Aγ chain, thus masking either a Lys or an Arg residue at the two extremes. © 1979.

引用

页码：7 / 11

页数：5

共 10 条

[1]

ABRAHAM E C, 1976, Hemoglobin, V1, P27, DOI 10.3109/03630267609031020

[2]

BETKE K, 1977, NATURE, V184, P18

[3] ABNORMAL HUMAN HAEMOGLOBINS - SEPARATION AND CHARACTERIZATION OF ALPHA AND BETA CHAINS BY CHROMATOGRAPHY AND DETERMINATION OF 2 NEW VARIANTS HB CHESAPEAKE AND HB J (BANGKOK) [J].